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[KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)

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CAB6128
  • Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)
  • Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)
  • Immunohistochemistry - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)
  • Immunoprecipitation - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)
$553.89

Description

Overview

Title:[KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)
Size:100µL
Code:CAB6128
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
GeneID:6599
SwissProt:Q92922
Synonyms:SMARCC1; BAF155; CRACC1; Rsc8; SRG3; SWI3; SWI/SNF complex subunit SMARCC1
Calculated MW:122kDa
Observed MW:155kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:BT-474, SW620, 293T
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)

Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (CAB6128) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.

Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)

Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (CAB6128) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)

Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (CAB6128) at dilution of 1:200 (40x lens).

Immunoprecipitation - [KO Validated] SMARCC1 Polyclonal Antibody (CAB6128)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (CAB6128). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (CAB6128) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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