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[KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

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CAB0887
  • Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
  • Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
  • Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
  • Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
  • Immunofluorescence - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
$553.89

Description

Overview

Title:[KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)
Size:100µL
Code:CAB0887
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide of human SMARCA4
GeneID:6597
SwissProt:P51532
Synonyms:SMARCA4; BAF190; BAF190A; BRG1; CSS4; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; transcription activator BRG1
Calculated MW:181kDa/184kDa
Observed MW:180kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:OVCAR3, HeLa
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

Western blot analysis of extracts of various cell lines, using SMARCA4 antibody (CAB0887) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

Western blot analysis of extracts from normal (control) and SMARCA4 knockout (KO) 293T cells, using SMARCA4 antibody (CAB0887) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody (CAB0887) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody (CAB0887) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] SMARCA4 Polyclonal Antibody (CAB0887)

Immunofluorescence analysis of HeLa cells using SMARCA4 antibody (CAB0887).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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