Description
Title: | [KO Validated] SGTA Polyclonal Antibody (CAB7306) | |
Size: | 100µL | |
Code: | CAB7306 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1). |
GeneID: | 6449 |
SwissProt: | O43765 |
Synonyms: | SGTA; SGT; alphaSGT; hSGT; small glutamine rich tetratricopeptide repeat containing alpha |
Calculated MW: | 34kDa |
Observed MW: | 38kDa |
Applications
Reactivity: | Human |
Tested Applications: | WB; IHC; IF |
Recommended Dilution: | WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting Immunohistochemistry Immunofluorescence |
Positive Samples: | HeLa, A-549, U-251MG |
Cellular Location: | N/A |
Antibody Images
Western blot analysis of extracts of various cell lines, using SGTA antibody (CAB7306) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. | |
Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (CAB7306) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. | |
Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (CAB7306) at dilution of 1:100 (40x lens). | |
Immunofluorescence analysis of A-549 cells using SGTA antibody (CAB7306). Blue: DAPI for nuclear staining. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IHC |
Application: |
IF |
Reactivity: |
Human |
Host Species: |
Rabbit |
Isotype: |
IgG |