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[KO Validated] SGTA Polyclonal Antibody (CAB7306)

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CAB7306
  • Western blot - [KO Validated] SGTA Polyclonal Antibody (CAB7306)
  • Western blot - [KO Validated] SGTA Polyclonal Antibody (CAB7306)
  • Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (CAB7306)
  • Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (CAB7306)
$553.89

Description

Overview

Title:[KO Validated] SGTA Polyclonal Antibody (CAB7306)
Size:100µL
Code:CAB7306
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
GeneID:6449
SwissProt:O43765
Synonyms:SGTA; SGT; alphaSGT; hSGT; small glutamine rich tetratricopeptide repeat containing alpha
Calculated MW:34kDa
Observed MW:38kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, A-549, U-251MG
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] SGTA Polyclonal Antibody (CAB7306)

Western blot analysis of extracts of various cell lines, using SGTA antibody (CAB7306) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] SGTA Polyclonal Antibody (CAB7306)

Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (CAB7306) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (CAB7306)

Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (CAB7306) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (CAB7306)

Immunofluorescence analysis of A-549 cells using SGTA antibody (CAB7306). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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