Description
Title: | [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794) | |
Size: | 100µL | |
Code: | CAB7794 | |
Host Species: | Rabbit | |
Purification: | Affinity purification | |
Isotype: | IgG |
Background
This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.
Immunogen Information
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2). |
GeneID: | 25939 |
SwissProt: | Q9Y3Z3 |
Synonyms: | SAMHD1; CHBL2; DCIP; HDDC1; MOP-5; SBBI88; deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
Calculated MW: | 63kDa/68kDa/72kDa |
Observed MW: | 72kDa |
Applications
Reactivity: | Human, Mouse, Rat |
Tested Applications: | WB; IF |
Recommended Dilution: | WB 1:500 - 1:2000; IF 1:50 - 1:100 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Application Key: | Western blotting Immunofluorescence |
Positive Samples: | HepG2, HeLa, MCF-7, Mouse spleen |
Cellular Location: | Nucleus |
Antibody Images
Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (CAB7794) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. | |
Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (CAB7794) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min. | |
Immunofluorescence analysis of A549 cells using SAMHD1 antibody (CAB7794). Blue: DAPI for nuclear staining. |
Additional Information
Antibody Type: |
Polyclonal |
Application: |
WB |
Application: |
IF |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |