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[KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)

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CAB7794
  • Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)
  • Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)
  • Immunofluorescence - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)
$553.89

Description

Overview

Title:[KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)
Size:100µL
Code:CAB7794
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).
GeneID:25939
SwissProt:Q9Y3Z3
Synonyms:SAMHD1; CHBL2; DCIP; HDDC1; MOP-5; SBBI88; deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Calculated MW:63kDa/68kDa/72kDa
Observed MW:72kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HepG2, HeLa, MCF-7, Mouse spleen
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)

Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (CAB7794) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)

Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (CAB7794) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Immunofluorescence - [KO Validated] SAMHD1 Polyclonal Antibody (CAB7794)

Immunofluorescence analysis of A549 cells using SAMHD1 antibody (CAB7794). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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