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[KO Validated] S100A6 Polyclonal Antibody (CAB4104)

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CAB4104
  • Western blot - [KO Validated] S100A6 Polyclonal Antibody (CAB4104)
  • Western blot - [KO Validated] S100A6 Polyclonal Antibody (CAB4104)
$553.89

Description

Overview

Title:[KO Validated] S100A6 Polyclonal Antibody (CAB4104)
Size:100µL
Code:CAB4104
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
GeneID:6277
SwissProt:P06703
Synonyms:S100A6; 2A9; 5B10; CABP; CACY; PRA; protein S100-A6
Calculated MW:10kDa
Observed MW:10kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HepG2, NIH/3T3, HT-29, Mouse lung, Mouse skeletal muscle
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus envelope, Peripheral membrane protein

Antibody Images

Western blot - [KO Validated] S100A6 Polyclonal Antibody (CAB4104)

Western blot analysis of extracts of various cell lines, using S100A6 antibody (CAB4104) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Western blot - [KO Validated] S100A6 Polyclonal Antibody (CAB4104)

Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using S100A6 antibody (CAB4104) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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