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[KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

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CAB1008
  • Western blot - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
  • Western blot - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
  • Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
  • Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
  • Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
  • Immunoprecipitation - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
€499.00

Description

Overview

Title:[KO Validated] ROCK1 Polyclonal Antibody (CAB1008)
Size:100µL
Code:CAB1008
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
GeneID:6093
SwissProt:Q13464
Synonyms:ROCK1; P160ROCK; ROCK-I; rho-associated protein kinase 1
Calculated MW:158kDa
Observed MW:158kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:293T, U-251MG, Jurkat, BT-474
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, bleb, centriole, centrosome, cytoskeleton, lamellipodium, microtubule organizing center, ruffle

Antibody Images

Western blot - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (CAB1008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (CAB1008) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody (CAB1008) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody (CAB1008) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody (CAB1008) at dilution of 1:100 (40x lens).

Immunoprecipitation - [KO Validated] ROCK1 Polyclonal Antibody (CAB1008)

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (CAB1008). Western blot was performed from the immunoprecipitate using ROCK1 antibody (CAB1008) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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