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[KO Validated] RHOC Polyclonal Antibody (CAB1062)

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CAB1062
  • Western blot - [KO Validated] RHOC Polyclonal Antibody (CAB1062)
  • Western blot - [KO Validated] RHOC Polyclonal Antibody (CAB1062)
  • Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (CAB1062)
$553.89

Description

Overview

Title:[KO Validated] RHOC Polyclonal Antibody (CAB1062)
Size:100µL
Code:CAB1062
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
GeneID:389
SwissProt:P08134
Synonyms:RHOC; ARH9; ARHC; H9; RHOH9; ras homolog family member C
Calculated MW:22kDa
Observed MW:22kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U2OS, Jurkat, A-549, PC-12
Cellular Location:Cell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor

Antibody Images

Western blot - [KO Validated] RHOC Polyclonal Antibody (CAB1062)

Western blot analysis of extracts of various cell lines, using RHOC antibody (CAB1062).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] RHOC Polyclonal Antibody (CAB1062)

Western blot analysis of extracts from normal (control) and RHOC knockout (KO) 293T cells, using RHOC antibody (CAB1062) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 300s.

Immunofluorescence - [KO Validated] RHOC Polyclonal Antibody (CAB1062)

Immunofluorescence analysis of HeLa cells using RHOC antibody (CAB1062). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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