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[KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)

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CAB1531
  • Western blot - [KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)
  • Western blot - [KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)
$553.89

Description

Overview

Title:[KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)
Size:100µL
Code:CAB1531
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).
GeneID:5576
SwissProt:P13861
Synonyms:PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit
Calculated MW:43kDa/45kDa
Observed MW:46kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle
Cellular Location:Cell membrane, Cytoplasm

Antibody Images

Western blot - [KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)

Western blot analysis of extracts of various cell lines, using PRKAR2A antibody (CAB1531) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] PRKAR2A Polyclonal Antibody (CAB1531)

Western blot analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using PRKAR2A antibody (CAB1531) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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