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[KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)

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CAB12491
  • Western blot - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)
  • Western blot - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)
  • Immunohistochemistry - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)
$553.89

Description

Overview

Title:[KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)
Size:100µL
Code:CAB12491
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human PRKAB1 (NP_006244.2).
GeneID:5564
SwissProt:Q9Y478
Synonyms:PRKAB1; AMPK; HAMPKb; 5'-AMP-activated protein kinase subunit beta-1
Calculated MW:30kDa
Observed MW:38kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:U-87MG, A-549, HeLa, Jurkat, HepG2
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)

Western blot analysis of extracts of various cell lines, using PRKAB1 antibody (CAB12491) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)

Western blot analysis of extracts from normal (control) and PRKAB1 knockout (KO) HeLa cells, using PRKAB1 antibody (CAB12491) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] PRKAB1 Polyclonal Antibody (CAB12491)

Immunohistochemistry of paraffin-embedded human esophagus using PRKAB1 antibody (CAB12491) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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