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[KO Validated] PRDX4 Polyclonal Antibody (CAB1486)

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CAB1486
  • Western blot - [KO Validated] PRDX4 Polyclonal Antibody (CAB1486)
  • Western blot - [KO Validated] PRDX4 Polyclonal Antibody (CAB1486)
$553.89

Description

Overview

Title:[KO Validated] PRDX4 Polyclonal Antibody (CAB1486)
Size:100µL
Code:CAB1486
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human PRDX4 (NP_006397.1).
GeneID:10549
SwissProt:Q13162
Synonyms:PRDX4; AOE37-2; AOE372; HEL-S-97n; PRX-4; peroxiredoxin-4
Calculated MW:30kDa
Observed MW:28kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:H460, PC-3, HeLa, MCF-7, Mouse testis
Cellular Location:Cytoplasm, Secreted

Antibody Images

Western blot - [KO Validated] PRDX4 Polyclonal Antibody (CAB1486)

Western blot analysis of extracts of various cell lines, using PRDX4 antibody (CAB1486) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] PRDX4 Polyclonal Antibody (CAB1486)

Western blot analysis of extracts from normal (control) and PRDX4 knockout (KO) 293T cells, using PRDX4 antibody (CAB1486) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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