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[KO Validated] POR Polyclonal Antibody (CAB8142)

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CAB8142
  • Western blot - [KO Validated] POR Polyclonal Antibody (CAB8142)
  • Western blot - [KO Validated] POR Polyclonal Antibody (CAB8142)
  • Immunohistochemistry - [KO Validated] POR Polyclonal Antibody (CAB8142)
$553.89

Description

Overview

Title:[KO Validated] POR Polyclonal Antibody (CAB8142)
Size:100µL
Code:CAB8142
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human POR (NP_000932.3).
GeneID:5447
SwissProt:P16435
Synonyms:POR; CPR; CYPOR; P450R; cytochrome p450 oxidoreductase
Calculated MW:76kDa
Observed MW:77kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:A-549, HeLa, MCF-7, 293T, Mouse liver, Mouse skin, Mouse kidney, Rat brain
Cellular Location:Endoplasmic reticulum membrane, Peripheral membrane protein

Antibody Images

Western blot - [KO Validated] POR Polyclonal Antibody (CAB8142)

Western blot analysis of extracts of various cell lines, using POR antibody (CAB8142) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] POR Polyclonal Antibody (CAB8142)

Western blot analysis of extracts from normal (control) and POR knockout (KO) HeLa cells, using POR antibody (CAB8142) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] POR Polyclonal Antibody (CAB8142)

Immunohistochemistry of paraffin-embedded human esophagus using POR antibody (CAB8142) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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