The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] POLE3 Polyclonal Antibody (CAB6469)

(No reviews yet) Write a Review
SKU:
CAB6469
  • Western blot - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)
  • Western blot - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)
  • Immunofluorescence - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)
  • Immunofluorescence - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)
$553.89

Description

Overview

Title:[KO Validated] POLE3 Polyclonal Antibody (CAB6469)
Size:100µL
Code:CAB6469
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3).
GeneID:54107
SwissProt:Q9NRF9
Synonyms:POLE3; CHARAC17; CHRAC17; YBL1; p17; DNA polymerase epsilon subunit 3
Calculated MW:16kDa
Observed MW:20-25kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HepG2, Mouse testis, Mouse bone marrow, Mouse spleen
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)

Western blot analysis of extracts of various cell lines, using POLE3 antibody (CAB6469) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Western blot - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)

Western blot analysis of extracts from normal (control) and POLE3 knockout (KO) 293T cells, using POLE3 antibody (CAB6469) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)

Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469).

Immunofluorescence - [KO Validated] POLE3 Polyclonal Antibody (CAB6469)

Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469). Green?gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose