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[KO Validated] PML Polyclonal Antibody (CAB1184)

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CAB1184
  • Western blot - [KO Validated] PML Polyclonal Antibody (CAB1184)
  • Western blot - [KO Validated] PML Polyclonal Antibody (CAB1184)
  • Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (CAB1184)
  • Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (CAB1184)
  • Immunofluorescence - [KO Validated] PML Polyclonal Antibody (CAB1184)
$553.89

Description

Overview

Title:[KO Validated] PML Polyclonal Antibody (CAB1184)
Size:100µL
Code:CAB1184
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).
GeneID:5371
SwissProt:P29590
Synonyms:PML; MYL; PP8675; RNF71; TRIM19; protein PML
Calculated MW:47-48kDa/62-97kDa
Observed MW:98kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:DU145
Cellular Location:Cytoplasm, Cytoplasmic side, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, PML body, Peripheral membrane protein, nucleolus, nucleoplasm

Antibody Images

Western blot - [KO Validated] PML Polyclonal Antibody (CAB1184)

Western blot analysis of extracts of various cell lines, using PML antibody (CAB1184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] PML Polyclonal Antibody (CAB1184)

Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (CAB1184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (CAB1184)

Immunohistochemistry of paraffin-embedded human gastric cancer using PML antibody (CAB1184) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (CAB1184)

Immunohistochemistry of paraffin-embedded human uterine cancer using PML antibody (CAB1184) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] PML Polyclonal Antibody (CAB1184)

Immunofluorescence analysis of HeLa cells using PML antibody (CAB1184). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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