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[KO Validated] PFKP Polyclonal Antibody (CAB7916)

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CAB7916
  • Western blot - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
  • Western blot - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
  • Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
  • Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
  • Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
  • Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)
$553.89

Description

Overview

Title:[KO Validated] PFKP Polyclonal Antibody (CAB7916)
Size:100µL
Code:CAB7916
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human PFKP
GeneID:5214
SwissProt:Q01813
Synonyms:PFKP; ATP-PFK; PFK-C; PFK-P; PFKF; phosphofructokinase, platelet
Calculated MW:85kDa
Observed MW:100kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:MCF-7, SKOV3, HeLa, NCI-H460, Mouse brain, Rat heart
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Western blot analysis of extracts of various cell lines, using PFKP antibody (CAB7916) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

Western blot - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Western blot analysis of extracts from normal (control) and PFKP knockout (KO) HeLa cells, using PFKP antibody (CAB7916) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Immunohistochemistry of paraffin-embedded mouse kidney using PFKP antibody (CAB7916) (40x lens).

Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Immunohistochemistry of paraffin-embedded rat brain using PFKP antibody (CAB7916) (40x lens).

Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Immunohistochemistry of paraffin-embedded rat kidney using PFKP antibody (CAB7916) (40x lens).

Immunohistochemistry - [KO Validated] PFKP Polyclonal Antibody (CAB7916)

Immunohistochemistry of paraffin-embedded mouse brain using PFKP antibody (CAB7916) (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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