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[KO Validated] PDCD4 Polyclonal Antibody (CAB2570)

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CAB2570
  • Western blot - [KO Validated] PDCD4 Polyclonal Antibody (CAB2570)
  • Western blot - [KO Validated] PDCD4 Polyclonal Antibody (CAB2570)
$553.89

Description

Overview

Title:[KO Validated] PDCD4 Polyclonal Antibody (CAB2570)
Size:100µL
Code:CAB2570
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).
GeneID:27250
SwissProt:Q53EL6
Synonyms:PDCD4; H731; programmed cell death 4
Calculated MW:50kDa/51kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, HeLa, HepG2, Mouse thymus
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] PDCD4 Polyclonal Antibody (CAB2570)

Western blot analysis of extracts of various cell lines, using PDCD4 antibody (CAB2570) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Western blot - [KO Validated] PDCD4 Polyclonal Antibody (CAB2570)

Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) HeLa cells, using PDCD4 antibody (CAB2570) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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