The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] PDCD10 Polyclonal Antibody (CAB15400)

(No reviews yet) Write a Review
SKU:
CAB15400
  • Western blot - [KO Validated] PDCD10 Polyclonal Antibody (CAB15400)
  • Western blot - [KO Validated] PDCD10 Polyclonal Antibody (CAB15400)
$553.89

Description

Overview

Title:[KO Validated] PDCD10 Polyclonal Antibody (CAB15400)
Size:100µL
Code:CAB15400
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
GeneID:11235
SwissProt:Q9BUL8
Synonyms:PDCD10; CCM3; TFAR15; programmed cell death 10
Calculated MW:24kDa
Observed MW:26kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:200 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:A-431, HepG2, Jurkat, MCF7, Mouse thymus, Mouse brain
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein

Antibody Images

Western blot - [KO Validated] PDCD10 Polyclonal Antibody (CAB15400)

Western blot analysis of extracts of various cell lines, using PDCD10 antibody (CAB15400) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] PDCD10 Polyclonal Antibody (CAB15400)

Western blot analysis of extracts from normal (control) and PDCD10 knockout (KO) HeLa cells, using PDCD10 antibody (CAB15400) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose