The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] PARK7 Polyclonal Antibody (CAB0987)

(No reviews yet) Write a Review
SKU:
CAB0987
  • Western blot - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)
  • Western blot - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)
  • Immunofluorescence - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)
$553.89

Description

Overview

Title:[KO Validated] PARK7 Polyclonal Antibody (CAB0987)
Size:100µL
Code:CAB0987
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).
GeneID:11315
SwissProt:Q99497
Synonyms:PARK7; DJ-1; DJ1; GATD2; HEL-S-67p; protein DJ-1
Calculated MW:19kDa
Observed MW:20kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, NIH/3T3, Jurkat
Cellular Location:Cell membrane, Cytoplasm, Lipid-anchor, Membrane raft, Mitochondrion, Nucleus

Antibody Images

Western blot - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)

Western blot analysis of extracts of various cell lines, using PARK7 antibody (CAB0987) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)

Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (CAB0987) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunofluorescence - [KO Validated] PARK7 Polyclonal Antibody (CAB0987)

Immunofluorescence analysis of HeLa cells using PARK7 antibody (CAB0987). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose