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[KO Validated] NUDT1 Polyclonal Antibody (CAB13330)

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CAB13330
  • Western blot - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)
  • Western blot - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)
  • Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)
  • Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)
$553.89

Description

Overview

Title:[KO Validated] NUDT1 Polyclonal Antibody (CAB13330)
Size:100µL
Code:CAB13330
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).
GeneID:4521
SwissProt:P36639
Synonyms:NUDT1; MTH1; nudix hydrolase 1
Calculated MW:17kDa/19kDa/20kDa/22kDa
Observed MW:18kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:SW480, K562, 22RV1, THP-1, HepG2, SKOV3, 293T
Cellular Location:Cytoplasm, Mitochondrion matrix, Nucleus

Antibody Images

Western blot - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)

Western blot analysis of extracts of various cell lines, using NUDT1 antibody (CAB13330) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Western blot - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)

Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody (CAB13330) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)

Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody (CAB13330) at dilution of 1:100 (40x lens).

Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (CAB13330)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug NUDT1 antibody (CAB13330). Western blot was performed from the immunoprecipitate using NUDT1 antibody (CAB13330) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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