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[KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)

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CAB7636
  • Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)
  • Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)
  • Immunohistochemistry - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)
  • Immunofluorescence - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)
$331.89

Description

Overview

Title:[KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)
Size:100µL
Code:CAB7636
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.

Immunogen Information

Immunogen:A synthetic peptide of human NOTCH1
GeneID:4851
SwissProt:P46531
Synonyms:NOTCH1; AOS5; AOVD1; TAN1; hN1; notch 1
Calculated MW:272kDa
Observed MW:300kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7, 293T, OVCAR3, Mouse thymus
Cellular Location:Cell membrane, Nucleus, Single-pass type I membrane protein

Antibody Images

Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)

Western blot analysis of extracts of various cell lines, using NOTCH1 antibody (CAB7636) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Western blot - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)

Western blot analysis of extracts from normal (control) and NOTCH1 knockout (KO) HeLa cells, using NOTCH1 antibody (CAB7636) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)

Immunohistochemistry of paraffin-embedded rat spleen using NOTCH1 antibody (CAB7636) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] NOTCH1 Polyclonal Antibody (CAB7636)

Immunofluorescence analysis of U2OS cells using NOTCH1 antibody (CAB7636) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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