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[KO Validated] NFKBIA Polyclonal Antibody (CAB11397)

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CAB11397
  • Western blot - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)
  • Western blot - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)
  • Immunofluorescence - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)
  • Immunoprecipitation - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)
$553.89

Description

Overview

Title:[KO Validated] NFKBIA Polyclonal Antibody (CAB11397)
Size:100µL
Code:CAB11397
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human NFKBIA (NP_065390.1).
GeneID:4792
SwissProt:P25963
Synonyms:NFKBIA; IKBA; MAD-3; NFKBI; NFKB inhibitor alpha
Calculated MW:35kDa
Observed MW:36kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:20 - 1:50; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:MCF-7, DU145, THP-1, A-549, Mouse spleen, Mouse thymus
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)

Western blot analysis of extracts of various cell lines, using NFKBIA antibody (CAB11397) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)

Western blot analysis of extracts from normal (control) and NFKBIA knockout (KO) 293T cells, using NFKBIA antibody (CAB11397) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunofluorescence - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)

Immunofluorescence analysis of HeLa cells using NFKBIA antibody (CAB11397). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] NFKBIA Polyclonal Antibody (CAB11397)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug NFKBIA antibody (CAB11397). Western blot was performed from the immunoprecipitate using NFKBIA antibody (CAB11397) at a dilition of 1:500.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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