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[KO Validated] NFKB1 Polyclonal Antibody (CAB6667)

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CAB6667
  • Western blot - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)
  • Western blot - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)
  • Immunohistochemistry - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)
  • Immunofluorescence - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)
$553.89

Description

Overview

Title:[KO Validated] NFKB1 Polyclonal Antibody (CAB6667)
Size:100µL
Code:CAB6667
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
GeneID:4790
SwissProt:P19838
Synonyms:NFKB1; CVID12; EBP-1; KBF1; NF-kB1; NF-kappa-B; NF-kappaB; NFKB-p105; NFKB-p50; NFkappaB; p105; p50; nuclear factor kappa B subunit 1
Calculated MW:85kDa/105kDa
Observed MW:120kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Raji, Mouse thymus
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody (CAB6667) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)

Western blot analysis of extracts from normal (control) and NFKB1 knockout (KO) HeLa cells, using NFKB1 antibody (CAB6667) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody (CAB6667) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] NFKB1 Polyclonal Antibody (CAB6667)

Immunofluorescence analysis of A549 cells using NFKB1 antibody (CAB6667).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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