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[KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)

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CAB8013
  • Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)
  • Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)
  • Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)
  • Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)
$553.89

Description

Overview

Title:[KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)
Size:100µL
Code:CAB8013
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
GeneID:4722
SwissProt:O75489
Synonyms:NDUFS3; CI-30; NADH:ubiquinone oxidoreductase core subunit S3
Calculated MW:14kDa/30kDa
Observed MW:30kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:MCF-7, SW620, HepG2, Mouse kidney, Mouse liver, Mouse heart, Rat liver, Rat heart
Cellular Location:Mitochondrion inner membrane

Antibody Images

Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)

Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (CAB8013) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)

Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (CAB8013) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)

Immunohistochemistry of paraffin-embedded human liver injury using NDUFS3 antibody (CAB8013) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (CAB8013)

Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (CAB8013) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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