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[KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)

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CAB12858
  • Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)
  • Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)
$553.89

Description

Overview

Title:[KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)
Size:100µL
Code:CAB12858
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1).
GeneID:4720
SwissProt:O75306
Synonyms:NDUFS2; CI-49; NADH:ubiquinone oxidoreductase core subunit S2
Calculated MW:51kDa/52kDa
Observed MW:49kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:3000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:LO2, HeLa, 293T, Mouse brain, Mouse kidney, Mouse spleen, Rat brain
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein

Antibody Images

Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)

Western blot analysis of extracts of various cell lines, using NDUFS2 antibody (CAB12858) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] NDUFS2 Polyclonal Antibody (CAB12858)

Western blot analysis of extracts from normal (control) and NDUFS2 knockout (KO) 293T cells, using NDUFS2 antibody (CAB12858) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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