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[KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)

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CAB15073
  • Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)
  • Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)
$553.89

Description

Overview

Title:[KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)
Size:100µL
Code:CAB15073
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1).
GeneID:4718
SwissProt:O95298
Synonyms:NDUFC2; B14.5b; CI-B14.5b; HLC-1; NADHDH2; NADH:ubiquinone oxidoreductase subunit C2
Calculated MW:10kDa/11kDa/13kDa/14kDa
Observed MW:14kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:293T, OVCAR3, HeLa, A-549, mouse stomach, mouse brain, mouse heart, rat kidney
Cellular Location:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein

Antibody Images

Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)

Western blot analysis of extracts of various cell lines, using NDUFC2 antibody (CAB15073) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Western blot - [KO Validated] NDUFC2 Polyclonal Antibody (CAB15073)

Western blot analysis of extracts from normal (control) and NDUFC2 knockout (KO) HeLa cells, using NDUFC2 antibody (CAB15073) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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