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[KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)

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CAB13820
  • Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)
  • Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)
$553.89

Description

Overview

Title:[KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)
Size:100µL
Code:CAB13820
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
GeneID:4710
SwissProt:O95168
Synonyms:NDUFB4; B15; CI-B15; NADH:ubiquinone oxidoreductase subunit B4
Calculated MW:14kDa/15kDa
Observed MW:15kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, HeLa, 293T, Mouse heart, Mouse kidney, Mouse liver
Cellular Location:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein

Antibody Images

Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)

Western blot analysis of extracts of various cell lines, using NDUFB4 antibody (CAB13820) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (CAB13820)

Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using NDUFB4 antibody (CAB13820) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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