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[KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)

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CAB14751
  • Western blot - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)
  • Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)
  • Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)
  • Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)
$553.89

Description

Overview

Title:[KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)
Size:100µL
Code:CAB14751
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
GeneID:4698
SwissProt:Q16718
Synonyms:NDUFA5; B13; CI-13KD-B; CI-13kB; NUFM; UQOR13; NADH:ubiquinone oxidoreductase subunit A5
Calculated MW:13kDa
Observed MW:Refer to figures

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:N/A
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein

Antibody Images

Western blot - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)

Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (CAB14751) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)

Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)

Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] NDUFA5 Polyclonal Antibody (CAB14751)

Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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