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[KO Validated] NBN Polyclonal Antibody (CAB0783)

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CAB0783
  • Western blot - [KO Validated] NBN Polyclonal Antibody (CAB0783)
  • Western blot - [KO Validated] NBN Polyclonal Antibody (CAB0783)
$553.89

Description

Overview

Title:[KO Validated] NBN Polyclonal Antibody (CAB0783)
Size:100µL
Code:CAB0783
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).
GeneID:4683
SwissProt:O60934
Synonyms:NBN; AT-V1; AT-V2; ATV; NBS; NBS1; P95; nibrin
Calculated MW:84kDa
Observed MW:110kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:1000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-87MG, HeLa, HepG2, mouse liver
Cellular Location:Chromosome, Nucleus, PML body, telomere

Antibody Images

Western blot - [KO Validated] NBN Polyclonal Antibody (CAB0783)

Western blot analysis of extracts of various cell lines, using NBN antibody (CAB0783) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] NBN Polyclonal Antibody (CAB0783)

Western blot analysis of extracts from normal (control) and NBN knockout (KO) HeLa cells, using NBN antibody (CAB0783) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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