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[KO Validated] MAPK6 Polyclonal Antibody (CAB15304)

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CAB15304
  • Western blot - [KO Validated] MAPK6 Polyclonal Antibody (CAB15304)
  • Western blot - [KO Validated] MAPK6 Polyclonal Antibody (CAB15304)
$553.89

Description

Overview

Title:[KO Validated] MAPK6 Polyclonal Antibody (CAB15304)
Size:100µL
Code:CAB15304
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).
GeneID:5597
SwissProt:Q16659
Synonyms:MAPK6; ERK3; HsT17250; PRKM6; p97MAPK; mitogen-activated protein kinase 6
Calculated MW:82kDa
Observed MW:120kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-251MG, A549, LO2, BxPC-3, 293T, Mouse brain
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] MAPK6 Polyclonal Antibody (CAB15304)

Western blot analysis of extracts of various cell lines, using MAPK6 antibody (CAB15304) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Western blot - [KO Validated] MAPK6 Polyclonal Antibody (CAB15304)

Western blot analysis of extracts from normal (control) and MAPK6 knockout (KO) HeLa cells, using MAPK6 antibody (CAB15304) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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