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[KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)

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CAB14781
  • Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)
  • Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)
$553.89

Description

Overview

Title:[KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)
Size:100µL
Code:CAB14781
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).
GeneID:6416
SwissProt:P45985
Synonyms:MAP2K4; JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; mitogen-activated protein kinase kinase 4
Calculated MW:44kDa/45kDa
Observed MW:44kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:SH-SY5Y, BT-474, MCF-7, mouse thymus, mouse liver, rat liver
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)

Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (CAB14781) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] MAP2K4 Polyclonal Antibody (CAB14781)

Western blot analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MAP2K4 antibody (CAB14781) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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