The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

(No reviews yet) Write a Review
SKU:
CAB13506
  • Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
  • Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
$553.89

Description

Overview

Title:[KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
Size:100µL
Code:CAB13506
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
GeneID:3958
SwissProt:P17931
Synonyms:LGALS3; CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2; galectin-3
Calculated MW:26kDa
Observed MW:31kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29, SGC-7901, HeLa, Mouse brain, Mouse kidney
Cellular Location:Cytoplasm, Nucleus, Secreted

Antibody Images

Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

Western blot analysis of extracts of various cell lines, using LGALS3 antibody (CAB13506) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (CAB13506) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose