[KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

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CAB13506
  • Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
  • Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

Overview

Title:[KO Validated] LGALS3 Polyclonal Antibody (CAB13506)
Size:100µL
Code:CAB13506
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).
GeneID:3958
SwissProt:P17931
Synonyms:LGALS3; CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2; galectin-3
Calculated MW:26kDa
Observed MW:31kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29, SGC-7901, HeLa, Mouse brain, Mouse kidney
Cellular Location:Cytoplasm, Nucleus, Secreted

Antibody Images

Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

Western blot analysis of extracts of various cell lines, using LGALS3 antibody (CAB13506) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] LGALS3 Polyclonal Antibody (CAB13506)

Western blot analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using LGALS3 antibody (CAB13506) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

[KO Validated] LGALS3 Polyclonal Antibody (CAB13506) Protein Information

UniProt Protein Function:Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Extracellular matrix; Cell surface

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: spliceosome; extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function:protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; epithelial cell differentiation; positive chemotaxis; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development

NCBI Summary:This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
UniProt Code:P17931
NCBI GenInfo Identifier:215274262
NCBI Gene ID:3958
NCBI Accession:P17931.5
UniProt Secondary Accession:P17931,Q16005, Q6IBA7, Q96J47, B2RC38,
UniProt Related Accession:P17931
Molecular Weight:
NCBI Full Name:Galectin-3
NCBI Synonym Full Names:lectin, galactoside-binding, soluble, 3
NCBI Official Symbol:LGALS3  
NCBI Official Synonym Symbols:L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2  
NCBI Protein Information:galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
UniProt Protein Name:Galectin-3
UniProt Synonym Protein Names:35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen
Protein Family:Galectin
UniProt Gene Name:LGALS3  
UniProt Entry Name:LEG3_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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