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[KO Validated] L1CAM Polyclonal Antibody (CAB8555)

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CAB8555
  • Western blot - [KO Validated] L1CAM Polyclonal Antibody (CAB8555)
  • Western blot - [KO Validated] L1CAM Polyclonal Antibody (CAB8555)
$553.89

Description

Overview

Title:[KO Validated] L1CAM Polyclonal Antibody (CAB8555)
Size:100µL
Code:CAB8555
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1).
GeneID:3897
SwissProt:P32004
Synonyms:L1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1; L1 cell adhesion molecule
Calculated MW:138kDa/139kDa/140kDa
Observed MW:200kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:SH-SY5Y, Mouse brain
Cellular Location:Cell membrane, Cell projection, Single-pass type I membrane protein, growth cone

Antibody Images

Western blot - [KO Validated] L1CAM Polyclonal Antibody (CAB8555)

Western blot analysis of extracts of various cell lines, using L1CAM antibody (CAB8555) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] L1CAM Polyclonal Antibody (CAB8555)

Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (CAB8555) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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