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[KO Validated] KRT8 Polyclonal Antibody (CAB1024)

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CAB1024
  • Western blot - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)
  • Western blot - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)
  • Immunohistochemistry - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)
  • Immunohistochemistry - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)
  • Immunofluorescence - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)
$553.89

Description

Overview

Title:[KO Validated] KRT8 Polyclonal Antibody (CAB1024)
Size:100µL
Code:CAB1024
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human KRT8 (NP_002264.1).
GeneID:3856
SwissProt:P05787
Synonyms:KRT8; CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; keratin 8
Calculated MW:53kDa/56kDa
Observed MW:53kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, NIH/3T3
Cellular Location:Cytoplasm, Nucleus, Nucleus matrix, nucleoplasm

Antibody Images

Western blot - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)

Western blot analysis of extracts of various cell lines, using KRT8 antibody (CAB1024) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)

Western blot analysis of extracts from normal (control) and KRT8 knockout (KO) HeLa cells, using KRT8 antibody (CAB1024) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)

Immunohistochemistry of paraffin-embedded human esophagus using KRT8 Antibody (CAB1024) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)

Immunohistochemistry of paraffin-embedded human esophagus using KRT8 Antibody (CAB1024) at dilution of 1:200 (40x lens).

Immunofluorescence - [KO Validated] KRT8 Polyclonal Antibody (CAB1024)

Immunofluorescence analysis of HeLa cells using KRT8 antibody (CAB1024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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