The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

(No reviews yet) Write a Review
SKU:
CAB7629
  • Western blot - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
  • Western blot - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
  • Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
  • Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
  • Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
$553.89

Description

Overview

Title:[KO Validated] ITGA2 Polyclonal Antibody (CAB7629)
Size:100µL
Code:CAB7629
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant protein of human ITGA2
GeneID:3673
SwissProt:P17301
Synonyms:ITGA2; BR; CD49B; GPIa; HPA-5; VLA-2; VLAA2; integrin alpha-2
Calculated MW:129kDa
Observed MW:150kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, HT-1080
Cellular Location:Membrane, Single-pass type I membrane protein

Antibody Images

Western blot - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (CAB7629) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Western blot - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (CAB7629) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (CAB7629) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (CAB7629) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] ITGA2 Polyclonal Antibody (CAB7629)

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (CAB7629) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose