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[KO Validated] IRF3 Polyclonal Antibody (CAB11118)

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CAB11118
  • Western blot - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)
  • Western blot - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)
  • Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)
  • Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)
  • Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)
$553.89

Description

Overview

Title:[KO Validated] IRF3 Polyclonal Antibody (CAB11118)
Size:100µL
Code:CAB11118
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
GeneID:3661
SwissProt:Q14653
Synonyms:IRF3; IIAE7; interferon regulatory factor 3
Calculated MW:12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW:56kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF; IP; ChIP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; ChIP 1:20 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, HeLa, Jurkat, HepG2
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)

Western blot analysis of extracts of various cell lines, using IRF3 antibody (CAB11118) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)

Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (CAB11118) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)

Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (CAB11118) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)

Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (CAB11118) at dilution of 1:100 (40x lens).

Chromatin Immunoprecipitation - [KO Validated] IRF3 Polyclonal Antibody (CAB11118)

Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (CAB11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Application:
ChIP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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