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[KO Validated] IKBKG Polyclonal Antibody (CAB12536)

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CAB12536
  • Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
  • Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
  • Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
$553.89

Description

Overview

Title:[KO Validated] IKBKG Polyclonal Antibody (CAB12536)
Size:100µL
Code:CAB12536
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKBKG (NP_003630.1).
GeneID:8517
SwissProt:Q9Y6K9
Synonyms:IKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9; NF-kappa-B essential modulator
Calculated MW:36kDa/48kDa/55kDa
Observed MW:48kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:A-549, Mouse liver
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Western blot analysis of extracts of various cell lines, using IKBKG Antibody (CAB12536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Western blot analysis of extracts from normal (control) and IKBKG knockout (KO) 293T cells, using IKBKG antibody (CAB12536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKBKG antibody (CAB12536). Western blot was performed from the immunoprecipitate using IKBKG antibody (CAB12536) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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