[KO Validated] IKBKG Polyclonal Antibody (CAB12536)

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CAB12536
  • Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
  • Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
  • Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Overview

Title:[KO Validated] IKBKG Polyclonal Antibody (CAB12536)
Size:100µL
Code:CAB12536
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKBKG (NP_003630.1).
GeneID:8517
SwissProt:Q9Y6K9
Synonyms:IKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9; NF-kappa-B essential modulator
Calculated MW:36kDa/48kDa/55kDa
Observed MW:48kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:A-549, Mouse liver
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Western blot analysis of extracts of various cell lines, using IKBKG Antibody (CAB12536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Western blot analysis of extracts from normal (control) and IKBKG knockout (KO) 293T cells, using IKBKG antibody (CAB12536) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunoprecipitation - [KO Validated] IKBKG Polyclonal Antibody (CAB12536)

Immunoprecipitation analysis of 200ug extracts of A549 cells using 3ug IKBKG antibody (CAB12536). Western blot was performed from the immunoprecipitate using IKBKG antibody (CAB12536) at a dilition of 1:1000.

[KO Validated] IKBKG Polyclonal Antibody (CAB12536) Protein Information

UniProt Protein Function:Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
NCBI Summary:This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
UniProt Code:Q9Y6K9
NCBI GenInfo Identifier:6685695
NCBI Gene ID:8517
NCBI Accession:Q9Y6K9.2
UniProt Secondary Accession:Q9Y6K9,Q7LBY6, Q7Z7F1,
UniProt Related Accession:Q9Y6K9
Molecular Weight:36,953 Da
NCBI Full Name:NF-kappa-B essential modulator
NCBI Synonym Full Names:inhibitor of nuclear factor kappa B kinase subunit gamma
NCBI Official Symbol:IKBKG  
NCBI Official Synonym Symbols:IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma  
NCBI Protein Information:NF-kappa-B essential modulator
UniProt Protein Name:NF-kappa-B essential modulator
UniProt Synonym Protein Names:FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier
UniProt Gene Name:IKBKG  
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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