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[KO Validated] IFITM3 Polyclonal Antibody (CAB13070)

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CAB13070
  • Western blot - [KO Validated] IFITM3 Polyclonal Antibody (CAB13070)
  • Western blot - [KO Validated] IFITM3 Polyclonal Antibody (CAB13070)
$553.89

Description

Overview

Title:[KO Validated] IFITM3 Polyclonal Antibody (CAB13070)
Size:100µL
Code:CAB13070
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).
GeneID:10410
SwissProt:Q01628
Synonyms:IFITM3; 1-8U; DSPA2b; IP15; interferon-induced transmembrane protein 3
Calculated MW:14kDa
Observed MW:15kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa
Cellular Location:Cell membrane, Late endosome membrane, Lysosome membrane, Single-pass type II membrane protein

Antibody Images

Western blot - [KO Validated] IFITM3 Polyclonal Antibody (CAB13070)

Western blot analysis of extracts of HeLa cells, using IFITM3 antibody (CAB13070) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] IFITM3 Polyclonal Antibody (CAB13070)

Western blot analysis of extracts from normal (control) and IFITM3 knockout (KO) HeLa cells, using IFITM3 antibody (CAB13070) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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