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[KO Validated] IDE Polyclonal Antibody (CAB1630)

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CAB1630
  • Western blot - [KO Validated] IDE Polyclonal Antibody (CAB1630)
  • Western blot - [KO Validated] IDE Polyclonal Antibody (CAB1630)
  • Immunofluorescence - [KO Validated] IDE Polyclonal Antibody (CAB1630)
$553.89

Description

Overview

Title:[KO Validated] IDE Polyclonal Antibody (CAB1630)
Size:100µL
Code:CAB1630
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
GeneID:3416
SwissProt:P14735
Synonyms:IDE; INSULYSIN; insulin-degrading enzyme
Calculated MW:54kDa/117kDa
Observed MW:118kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:10 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:DU145, Mouse liver
Cellular Location:Cell membrane, Cytoplasm, Secreted

Antibody Images

Western blot - [KO Validated] IDE Polyclonal Antibody (CAB1630)

Western blot analysis of extracts of various cell lines, using IDE antibody (CAB1630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).

Western blot - [KO Validated] IDE Polyclonal Antibody (CAB1630)

Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibody (CAB1630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunofluorescence - [KO Validated] IDE Polyclonal Antibody (CAB1630)

Immunofluorescence analysis of A549 cells using IDE antibody (CAB1630). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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