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[KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

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CAB16332
  • Western blot - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
  • Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
  • Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
  • Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
  • Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
  • Immunofluorescence - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
$553.89

Description

Overview

Title:[KO Validated] HSPB1 Polyclonal Antibody (CAB16332)
Size:100µL
Code:CAB16332
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant protein of human HSPB1
GeneID:3315
SwissProt:P04792
Synonyms:HSPB1; CMT2F; HEL-S-102; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27; heat shock protein beta-1
Calculated MW:N/A
Observed MW:25kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:N/A
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody (CAB16332) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody (CAB16332) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] HSPB1 Polyclonal Antibody (CAB16332)

Immunofluorescence analysis of HeLa cells using HSPB1 antibody (CAB16332) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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