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[KO Validated] HPRT1 Polyclonal Antibody (CAB5692)

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CAB5692
  • Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)
  • Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)
  • Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)
  • Immunofluorescence - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)
$553.89

Description

Overview

Title:[KO Validated] HPRT1 Polyclonal Antibody (CAB5692)
Size:100µL
Code:CAB5692
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
GeneID:3251
SwissProt:P00492
Synonyms:HPRT1; HGPRT; HPRT; hypoxanthine phosphoribosyltransferase 1
Calculated MW:24kDa
Observed MW:27kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:10 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:MCF-7, HL-60, SW480, 293T, HeLa, Mouse brain, Mouse liver
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)

Western blot analysis of extracts of various cell lines, using HPRT1 antibody (CAB5692) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)

Western blot analysis of extracts of various cells, using HPRT1 antibody (CAB5692) at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Western blot - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)

Western blot analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT1 antibody (CAB5692) at 1:1000 dilution.

Immunofluorescence - [KO Validated] HPRT1 Polyclonal Antibody (CAB5692)

Immunofluorescence analysis of U2OS cells using HPRT1 antibody (CAB5692). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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