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[KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

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CAB1162
  • Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
  • Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
€499.00

Description

Overview

Title:[KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)
Size:100µL
Code:CAB1162
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA2B1 (NP_002128.1).
GeneID:3181
SwissProt:P22626
Synonyms:HNRNPA2B1; HNRNPA2; HNRNPB1; HNRPA2; HNRPA2B1; HNRPB1; IBMPFD2; RNPA2; SNRPB1; heterogeneous nuclear ribonucleoprotein A2/B1
Calculated MW:36kDa/37kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, BxPC-3, PC-3, Mouse spleen
Cellular Location:Cytoplasm, Nucleus, nucleoplasm

Antibody Images

Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Western blot analysis of extracts of various cell lines, using HNRNPA2B1 antibody (CAB1162) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Western blot analysis of extracts from normal (control) and HNRNPA2B1 knockout (KO) 293T cells, using HNRNPA2B1 antibody (CAB1162) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded human colon using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded human kidney using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded human stomach using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:200 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded mouse brain using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded rat brain using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded rat kidney using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HNRNPA2B1 Polyclonal Antibody (CAB1162)

Immunohistochemistry of paraffin-embedded rat liver using HNRNPA2B1 Antibody (CAB1162) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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