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[KO Validated] HINT1 Polyclonal Antibody (CAB10221)

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CAB10221
  • Western blot - [KO Validated] HINT1 Polyclonal Antibody (CAB10221)
  • Western blot - [KO Validated] HINT1 Polyclonal Antibody (CAB10221)
£424.15

Description

Overview

Title:[KO Validated] HINT1 Polyclonal Antibody (CAB10221)
Size:100µL
Code:CAB10221
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif. This gene is considered a tumor suppressor gene. In addition, mutations in this gene can cause autosomal recessive neuromyotonia and axonal neuropathy. There are several related pseudogenes on chromosome 7. Several transcript variants have been observed.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HINT1 (NP_005331.1).
GeneID:3094
SwissProt:P49773
Synonyms:HINT1; HINT; NMAN; PKCI-1; PRKCNH1; histidine triad nucleotide-binding protein 1
Calculated MW:13kDa
Observed MW:14kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:22Rv1, HeLa, H460, K-562, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] HINT1 Polyclonal Antibody (CAB10221)

Western blot analysis of extracts of various cell lines, using HINT1 antibody (CAB10221) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] HINT1 Polyclonal Antibody (CAB10221)

Western blot analysis of extracts from normal (control) and HINT1 knockout (KO) HeLa cells, using HINT1 antibody (CAB10221) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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