[KO Validated] HELLS Polyclonal Antibody (CAB5831)

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CAB5831
  • Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] HELLS Polyclonal Antibody (CAB5831)

Overview

Title:[KO Validated] HELLS Polyclonal Antibody (CAB5831)
Size:100µL
Code:CAB5831
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
GeneID:3070
SwissProt:Q9NRZ9
Synonyms:HELLS; ICF4; LSH; Nbla10143; PASG; SMARCA6; lymphoid-specific helicase
Calculated MW:14kDa/36-39kDa/81-97kDa
Observed MW:120kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:MCF-7, A549, 293T, HepG2, Mouse thymus
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Western blot analysis of extracts of various cell lines, using HELLS antibody (CAB5831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (CAB5831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (CAB5831) at dilution of 1:100 (40x lens).

Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (CAB5831). Western blot was performed from the immunoprecipitate using HELLS antibody (CAB5831) at a dilition of 1:1000.

[KO Validated] HELLS Polyclonal Antibody (CAB5831) Protein Information

UniProt Protein Function:HELLS: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. By concanavalin-A in peripheral blood leukocytes. Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Belongs to the SNF2/RAD54 helicase family. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.6.1.-; EC 3.6.4.-; Helicase; Apoptosis

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: centric heterochromatin; nucleus; chromosome, pericentric region

Molecular Function:protein binding; DNA binding; helicase activity; ATP binding

Biological Process: mitosis; lymphocyte proliferation; transcription, DNA-dependent; methylation-dependent chromatin silencing; maintenance of DNA methylation; cell division; multicellular organismal development; centric heterochromatin formation

NCBI Summary:This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]
UniProt Code:Q9NRZ9
NCBI GenInfo Identifier:74761670
NCBI Gene ID:3070
NCBI Accession:Q9NRZ9.1
UniProt Secondary Accession:Q9NRZ9,Q3LID1, Q6I7N7, Q76H76, Q76H77, Q76H78, Q76H79 Q76H80, Q76H81, Q7Z397, Q7Z5X2, B2RB41,
UniProt Related Accession:Q9NRZ9
Molecular Weight:838
NCBI Full Name:Lymphoid-specific helicase
NCBI Synonym Full Names:helicase, lymphoid-specific
NCBI Official Symbol:HELLS  
NCBI Official Synonym Symbols:LSH; PASG; SMARCA6; Nbla10143  
NCBI Protein Information:lymphoid-specific helicase; HELLS; proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6
UniProt Protein Name:Lymphoid-specific helicase
UniProt Synonym Protein Names:Proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6
Protein Family:Lymphoid-specific helicase
UniProt Gene Name:HELLS  
UniProt Entry Name:HELLS_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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