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[KO Validated] HELLS Polyclonal Antibody (CAB5831)

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SKU:
CAB5831
  • Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
  • Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (CAB5831)
$553.89

Description

Overview

Title:[KO Validated] HELLS Polyclonal Antibody (CAB5831)
Size:100µL
Code:CAB5831
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2).
GeneID:3070
SwissProt:Q9NRZ9
Synonyms:HELLS; ICF4; LSH; Nbla10143; PASG; SMARCA6; lymphoid-specific helicase
Calculated MW:14kDa/36-39kDa/81-97kDa
Observed MW:120kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:MCF-7, A549, 293T, HepG2, Mouse thymus
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Western blot analysis of extracts of various cell lines, using HELLS antibody (CAB5831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (CAB5831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (CAB5831) at dilution of 1:100 (40x lens).

Immunoprecipitation - [KO Validated] HELLS Polyclonal Antibody (CAB5831)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HELLS antibody (CAB5831). Western blot was performed from the immunoprecipitate using HELLS antibody (CAB5831) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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