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[KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

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CAB5829
  • Western blot - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
  • Western blot - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
  • Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
  • Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
  • Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
$553.89

Description

Overview

Title:[KO Validated] HDAC8 Polyclonal Antibody (CAB5829)
Size:100µL
Code:CAB5829
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
GeneID:55869
SwissProt:Q9BY41
Synonyms:HDAC8; CDA07; CDLS5; HD8; HDACL1; MRXS6; RPD3; WTS; histone deacetylase 8
Calculated MW:15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa
Observed MW:42kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, Mouse lung
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (CAB5829) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (CAB5829) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (CAB5829)

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (CAB5829) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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