The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] HADHA Polyclonal Antibody (CAB5346)

(No reviews yet) Write a Review
SKU:
CAB5346
  • Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)
  • Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)
  • Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)
  • Immunofluorescence - [KO Validated] HADHA Polyclonal Antibody (CAB5346)
  • Immunoprecipitation - [KO Validated] HADHA Polyclonal Antibody (CAB5346)
$553.89

Description

Overview

Title:[KO Validated] HADHA Polyclonal Antibody (CAB5346)
Size:100µL
Code:CAB5346
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
GeneID:3030
SwissProt:P40939
Synonyms:HADHA; ECHA; GBP; HADH; LCEH; LCHAD; MTPA; TP-ALPHA; trifunctional enzyme subunit alpha, mitochondrial
Calculated MW:28kDa/83kDa
Observed MW:82kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:K-562, THP-1, SW480, PC-3, HeLa, Mouse intestine, Mouse heart, Mouse adipose
Cellular Location:Mitochondrion

Antibody Images

Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)

Western blot analysis of extracts of various cell lines, using HADHA antibody (CAB5346) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)

Western blot analysis of extracts of various cells, using HADHA antibody (CAB5346) at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Western blot - [KO Validated] HADHA Polyclonal Antibody (CAB5346)

Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (CAB5346) at 1:1000 dilution.

Immunofluorescence - [KO Validated] HADHA Polyclonal Antibody (CAB5346)

Immunofluorescence analysis of U2OS cells using HADHA antibody (CAB5346). Blue: DAPI for nuclear staining.

Immunoprecipitation - [KO Validated] HADHA Polyclonal Antibody (CAB5346)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug HADHA antibody (CAB5346). Western blot was performed from the immunoprecipitate using HADHA antibody (CAB5346) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose