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[KO Validated] GSS Polyclonal Antibody (CAB14535)

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CAB14535
  • Western blot - [KO Validated] GSS Polyclonal Antibody (CAB14535)
  • Western blot - [KO Validated] GSS Polyclonal Antibody (CAB14535)
$553.89

Description

Overview

Title:[KO Validated] GSS Polyclonal Antibody (CAB14535)
Size:100µL
Code:CAB14535
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human GSS (NP_000169.1).
GeneID:2937
SwissProt:P48637
Synonyms:GSS; GSHS; HEL-S-64p; HEL-S-88n; glutathione synthetase
Calculated MW:40kDa/52kDa
Observed MW:54kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:DU145, K-562, 293T, Mouse kidney, Mouse spleen, Mouse brain, Rat kidney
Cellular Location:N/A

Antibody Images

Western blot - [KO Validated] GSS Polyclonal Antibody (CAB14535)

Western blot analysis of extracts of various cell lines, using GSS antibody (CAB14535) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Western blot - [KO Validated] GSS Polyclonal Antibody (CAB14535)

Western blot analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using GSS antibody (CAB14535) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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