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[KO Validated] GNAI3 Polyclonal Antibody (CAB13307)

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CAB13307
  • Western blot - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)
  • Western blot - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)
  • Immunoprecipitation - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)
$553.89

Description

Overview

Title:[KO Validated] GNAI3 Polyclonal Antibody (CAB13307)
Size:100µL
Code:CAB13307
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).
GeneID:2773
SwissProt:P08754
Synonyms:GNAI3; 87U6; ARCND1; G protein subunit alpha i3
Calculated MW:40kDa
Observed MW:45kDa

Applications

Reactivity:Human
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:LO2, MCF-7, HL-60, HT-1080
Cellular Location:Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center

Antibody Images

Western blot - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)

Western blot analysis of extracts of various cell lines, using GNAI3 antibody (CAB13307) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)

Western blot analysis of extracts from normal (control) and GNAI3 knockout (KO) HeLa cells, using GNAI3 antibody (CAB13307) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunoprecipitation - [KO Validated] GNAI3 Polyclonal Antibody (CAB13307)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GNAI3 antibody (CAB13307). Western blot was performed from the immunoprecipitate using GNAI3 antibody (CAB13307) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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