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[KO Validated] GIT1 Polyclonal Antibody (CAB15437)

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CAB15437
  • Western blot - [KO Validated] GIT1 Polyclonal Antibody (CAB15437)
  • Western blot - [KO Validated] GIT1 Polyclonal Antibody (CAB15437)
£424.15

Description

Overview

Title:[KO Validated] GIT1 Polyclonal Antibody (CAB15437)
Size:100µL
Code:CAB15437
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2).
GeneID:28964
SwissProt:Q9Y2X7
Synonyms:GIT1; GIT ArfGAP 1
Calculated MW:19kDa/84kDa/85kDa
Observed MW:100kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:U-251MG, A-549, A375, Mouse heart, Rat testis
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] GIT1 Polyclonal Antibody (CAB15437)

Western blot analysis of extracts of various cell lines, using GIT1 antibody (CAB15437) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] GIT1 Polyclonal Antibody (CAB15437)

Western blot analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody (CAB15437) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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