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[KO Validated] FUS Polyclonal Antibody (CAB5921)

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CAB5921
  • Western blot - [KO Validated] FUS Polyclonal Antibody (CAB5921)
  • Western blot - [KO Validated] FUS Polyclonal Antibody (CAB5921)
  • Immunofluorescence - [KO Validated] FUS Polyclonal Antibody (CAB5921)
  • Immunofluorescence - [KO Validated] FUS Polyclonal Antibody (CAB5921)
$553.89

Description

Overview

Title:[KO Validated] FUS Polyclonal Antibody (CAB5921)
Size:100µL
Code:CAB5921
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
GeneID:2521
SwissProt:P35637
Synonyms:FUS; ALS6; ETM4; FUS1; HNRNPP2; POMP75; TLS; RNA-binding protein FUS
Calculated MW:53kDa
Observed MW:70kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:U-937, Jurkat
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] FUS Polyclonal Antibody (CAB5921)

Western blot analysis of extracts of various cell lines, using FUS antibody (CAB5921) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] FUS Polyclonal Antibody (CAB5921)

Western blot analysis of extracts from normal (control) and FUS knockout (KO) 293T cells, using FUS antibody (CAB5921) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - [KO Validated] FUS Polyclonal Antibody (CAB5921)

Immunofluorescence analysis of C6 cells using FUS antibody (CAB5921) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] FUS Polyclonal Antibody (CAB5921)

Immunofluorescence analysis of NIH/3T3 cells using FUS antibody (CAB5921) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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